Diffusion

Learning Objectives

After completing the lab, the student will be able to:

  1. Explain or define the term diffusion.
  2. Explain how different media affect the rate of diffusion.

Activity 1: Pre-Assessment

  1. What happens when an air freshener is sprayed in a corner? What is the name of the process that causes the molecules to move?
  2. Do you think that the rate of the air freshener molecules moving would change if the room temperature was warmer or colder? Why or why not?
  3. Discuss the answers to questions 1 and 2 with the class.

Activity 1: Diffusion

The movement of molecules from a higher concentrated area to a wider and less concentrated area is referred to as diffusion. For example, you can smell the aroma of food flowing through the atmosphere as you walk towards a cafeteria. Molecules collide with each other and are in constant motion because of their kinetic energy. This activity propels molecules to move where there is a less concentrated area. Therefore, the net movement of molecules is always from a tightly concentrated area to a less tightly packed area. Osmosis is the process of water diffusion through a selectively permeable membrane. In body systems, various constituents such as gases, liquids, and solids are dissolved in water when they flow through the cell membrane from a highly concentrated place to a less concentrated area in bodily systems. In a solution, the dissolved substance is called the solute and the substance in which the solute is dissolved is called the solvent.

Diffusion is the movement of molecules from an area where the molecule is highly concentrated to an area of low concentration, as illustrated in Figure 6.1. The rate of diffusion is dependent upon the temperature of a system, molecular size, and the medium through which diffusion is occurring (i.e., semi-solid, liquid, air). In this activity, we will be observing the diffusion of a dye through a beaker of water and through agar (a gelatinous substance), diffusion as a function of temperature, and diffusion as a function of molecular weight.

Illustration of the movement of molecules in two beakers of liquid showing how the molecules more from areas of higher concentration, where they are closer together, to areas of lower concentration, where they are more spread out.
Figure 6.1: In diffusion, molecules move from areas of high concentration to areas of low concentration.

Safety Precautions

  • Inform your teacher immediately of any broken glassware, as it could cause injuries.
  • Clean up any spilled water or other fluids to prevent other people from slipping.
  • Be careful with the dye as it can stain your clothes, and it should not be ingested.
  • Wash your hands with soap and water after completion of the activity.

For this activity, you will need the following:

  • Three 250 mL beakers
  • Water
  • Food coloring
  • Agar plates
  • Potassium permanganate
  • Congo red
  • Methylene blue
  • Thermometer
  • Incubator
  • Refrigerator
  • Clock or timer

For this activity, you will work in groups of four.

Structured Inquiry

Step 1: Measure 200 mL of room temperature water in a beaker. Put three drops of food coloring into the water. Time how long it takes for the dye to completely diffuse throughout the water. Record the time and describe in your notebook what you observe. Create a data table for your observations.

Step 2: Hypothesize/Predict: Predict what would happen to the rate of diffusion if you had beakers with both very hot and very cold water in them. Add your predictions to the data table you created in step 1.

Step 3: Student-led Planning: Determine how diffusion of the food color would be affected when the water is either very hot or very cold. Use a thermometer and record the temperature for each. Use a timer to measure how long it takes for complete diffusion to occur in all scenarios.

Step 4: Critical Analysis: Create a graph that shows how the diffusion rate is affected because of temperature change. Are the predictions you made in step 2 supported by your data? Why or why not? What methods could you use to improve your results? Discuss with your group and then write your answers in your notebook.

Guided Inquiry

Step 1: Gather four agar plates and the three dyes, provided by your instructor, that differ in molecular size: Congo red (mol. wt. 696.66 g/mol), methylene blue (319.85 g/mol), and potassium permanganate (mol. wt. 158.03).

Step 2: Hypothesize/Predict: How would the rate of diffusion of a molecule through a gel compare to its rate of diffusion through water? How would the rate of diffusion differ between molecules of different molecular sizes? Write your ideas in your notebook.

Step 3: Student-led planning: Use 1 plate for determining how molecular size affects diffusion using the 3 dyes. Determine how best to measure movement of the dye in an agar plate. Be sure to keep the dyes far enough apart so that they do not touch once they start diffusing. Get your instructor’s approval before proceeding with the experiment. Measure the distance that the dye spreads in 20-minute intervals for 1 hour.

Step 4: Examine the effect of temperature on the rate of diffusion for 1 dye of your choosing. With your group, determine 3 temperatures that would be appropriate. Measure the diameter of the dye spread for each. Write the results in your notebook.

Step 5: Critical Analysis: Rank all 3 dyes in terms of diffusion rate. What was the relationship between diffusion rate and molecular size? What is the relationship between temperature and diffusion rate? Discuss your answers with your group and write them in your notebook.

Assessments

  1. In a system, there is a concentration of molecules. However, on the outside, there is little to no concentration of this particular molecule. In which direction would the molecules be moving more so than the other direction?
  2. Diffusion is affected by what factors?
  3. Dye tends to move faster in warmer temperatures. Why is this?

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Lab Manual for Biology Part I Copyright © 2022 by LOUIS: The Louisiana Library Network is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, except where otherwise noted.

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